AIR: Turn off any fans, A/Cs, or heaters.

BODY: Wear gloves and a mask. 

WORKSTATION: Clean your workstation with a spray bottle with 70% isopropyl alcohol.

BAG: Wipe your bag down with an alcohol wipe or alcohol spray bottle. Do not wipe or get alcohol inside the air filter patch. 

INJECTION PORT: Leave a fresh alcohol wipe or paper towel with isopropyl alcohol over the injection port until you are ready to inject.

Attach the sterile needle to your syringe. Flame sterilize your syringe needle with a lighter or flame and allow it to cool.

Carefully insert your syringe needle into the injection port and inject the bag with 10 CCs of liquid culture or spores. 

Carefully pull the needle out the injection port and place the cap safely back on. Dispose of the needle in a designated receptacle. 

Cover the injection port with clean scotch tape to protect the bag from outside contamination. You may also place the alcohol wipe back on the injection port and allow the injection port to self-heal. 

Store the bag in a semi-dark room with no direct sunlight, off the ground, at about 70-78 degrees Fahrenheit. Pro Tip: keeping your environment at 75-78 degrees Fahrenheit will result in faster rates of colonization.

 Store your bag for about 1-3 weeks. (strain times vary)